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sheep polyclonal anti trem2  (R&D Systems)


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    R&D Systems sheep polyclonal anti trem2
    Sheep Polyclonal Anti Trem2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sheep polyclonal anti trem2/product/R&D Systems
    Average 96 stars, based on 122 article reviews
    sheep polyclonal anti trem2 - by Bioz Stars, 2026-06
    96/100 stars

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    sheep polyclonal anti trem2  (R&D Systems)
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    R&D Systems sheep polyclonal anti trem2
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    sheep anti trem2 polyclonal antibody  (R&D Systems)
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    (A) Related to . Immunostaining shows representative images of neurons treated with mPFFs and 2.8 µM MTK458. Primary hippocampal neurons were seeded for immunostaining, treated with mPFFs (0.5 µg/mL) and MTK458 as in (E), and on DIV14 fixed for immunostaining with antibodies against pS129 α-syn, NfH and TUJ1. Scale bar, 50 µm. (B) Mice were challenged with striatal injection of PFFs of PBS. After 2 weeks, ipsilateral or contralateral striatum brain pieces were harvested for sequential extraction with buffers containing increasing amounts of detergent. NP-40 insoluble fractions were analyzed by immunoblot. (C-D) Quantification of (B) is shown. (E-G) Mice were challenged with striatal injection of PBS or PFF, and then dosed with MTK458 for 6 months. Mice were analyzed for <t>TREM2</t> in the ipsilateral striatum (E), plasma CXCL1 (F), and plasma IL-6 (G) levels. (H) Similar experiment to , except that mice were sacrificed 180 days after PFF injection and MTK458 dosing (QD, PO) at the indicated concentrations. (I-J) Quantification of (H) is shown. Mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p<0.0001, n.s., not significant.
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    (A) Related to . Immunostaining shows representative images of neurons treated with mPFFs and 2.8 µM MTK458. Primary hippocampal neurons were seeded for immunostaining, treated with mPFFs (0.5 µg/mL) and MTK458 as in (E), and on DIV14 fixed for immunostaining with antibodies against pS129 α-syn, NfH and TUJ1. Scale bar, 50 µm. (B) Mice were challenged with striatal injection of PFFs of PBS. After 2 weeks, ipsilateral or contralateral striatum brain pieces were harvested for sequential extraction with buffers containing increasing amounts of detergent. NP-40 insoluble fractions were analyzed by immunoblot. (C-D) Quantification of (B) is shown. (E-G) Mice were challenged with striatal injection of PBS or PFF, and then dosed with MTK458 for 6 months. Mice were analyzed for <t>TREM2</t> in the ipsilateral striatum (E), plasma CXCL1 (F), and plasma IL-6 (G) levels. (H) Similar experiment to , except that mice were sacrificed 180 days after PFF injection and MTK458 dosing (QD, PO) at the indicated concentrations. (I-J) Quantification of (H) is shown. Mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p<0.0001, n.s., not significant.
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    af1729 rrid ab 354956 anti trem2 polyclonal sheep biotinconjugated  (R&D Systems)
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    (A) Related to . Immunostaining shows representative images of neurons treated with mPFFs and 2.8 µM MTK458. Primary hippocampal neurons were seeded for immunostaining, treated with mPFFs (0.5 µg/mL) and MTK458 as in (E), and on DIV14 fixed for immunostaining with antibodies against pS129 α-syn, NfH and TUJ1. Scale bar, 50 µm. (B) Mice were challenged with striatal injection of PFFs of PBS. After 2 weeks, ipsilateral or contralateral striatum brain pieces were harvested for sequential extraction with buffers containing increasing amounts of detergent. NP-40 insoluble fractions were analyzed by immunoblot. (C-D) Quantification of (B) is shown. (E-G) Mice were challenged with striatal injection of PBS or PFF, and then dosed with MTK458 for 6 months. Mice were analyzed for <t>TREM2</t> in the ipsilateral striatum (E), plasma CXCL1 (F), and plasma IL-6 (G) levels. (H) Similar experiment to , except that mice were sacrificed 180 days after PFF injection and MTK458 dosing (QD, PO) at the indicated concentrations. (I-J) Quantification of (H) is shown. Mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p<0.0001, n.s., not significant.
    Af1729 Rrid Ab 354956 Anti Trem2 Polyclonal Sheep Biotinconjugated, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    antibody anti trem2 sheep polyclonal r  (R&D Systems)
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    (A) Related to . Immunostaining shows representative images of neurons treated with mPFFs and 2.8 µM MTK458. Primary hippocampal neurons were seeded for immunostaining, treated with mPFFs (0.5 µg/mL) and MTK458 as in (E), and on DIV14 fixed for immunostaining with antibodies against pS129 α-syn, NfH and TUJ1. Scale bar, 50 µm. (B) Mice were challenged with striatal injection of PFFs of PBS. After 2 weeks, ipsilateral or contralateral striatum brain pieces were harvested for sequential extraction with buffers containing increasing amounts of detergent. NP-40 insoluble fractions were analyzed by immunoblot. (C-D) Quantification of (B) is shown. (E-G) Mice were challenged with striatal injection of PBS or PFF, and then dosed with MTK458 for 6 months. Mice were analyzed for <t>TREM2</t> in the ipsilateral striatum (E), plasma CXCL1 (F), and plasma IL-6 (G) levels. (H) Similar experiment to , except that mice were sacrificed 180 days after PFF injection and MTK458 dosing (QD, PO) at the indicated concentrations. (I-J) Quantification of (H) is shown. Mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p<0.0001, n.s., not significant.
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    biotinylated polyclonal sheep anti‐trem2 capture antibody  (R&D Systems)
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    (A) Related to . Immunostaining shows representative images of neurons treated with mPFFs and 2.8 µM MTK458. Primary hippocampal neurons were seeded for immunostaining, treated with mPFFs (0.5 µg/mL) and MTK458 as in (E), and on DIV14 fixed for immunostaining with antibodies against pS129 α-syn, NfH and TUJ1. Scale bar, 50 µm. (B) Mice were challenged with striatal injection of PFFs of PBS. After 2 weeks, ipsilateral or contralateral striatum brain pieces were harvested for sequential extraction with buffers containing increasing amounts of detergent. NP-40 insoluble fractions were analyzed by immunoblot. (C-D) Quantification of (B) is shown. (E-G) Mice were challenged with striatal injection of PBS or PFF, and then dosed with MTK458 for 6 months. Mice were analyzed for <t>TREM2</t> in the ipsilateral striatum (E), plasma CXCL1 (F), and plasma IL-6 (G) levels. (H) Similar experiment to , except that mice were sacrificed 180 days after PFF injection and MTK458 dosing (QD, PO) at the indicated concentrations. (I-J) Quantification of (H) is shown. Mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p<0.0001, n.s., not significant.
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    (A) Related to . Immunostaining shows representative images of neurons treated with mPFFs and 2.8 µM MTK458. Primary hippocampal neurons were seeded for immunostaining, treated with mPFFs (0.5 µg/mL) and MTK458 as in (E), and on DIV14 fixed for immunostaining with antibodies against pS129 α-syn, NfH and TUJ1. Scale bar, 50 µm. (B) Mice were challenged with striatal injection of PFFs of PBS. After 2 weeks, ipsilateral or contralateral striatum brain pieces were harvested for sequential extraction with buffers containing increasing amounts of detergent. NP-40 insoluble fractions were analyzed by immunoblot. (C-D) Quantification of (B) is shown. (E-G) Mice were challenged with striatal injection of PBS or PFF, and then dosed with MTK458 for 6 months. Mice were analyzed for TREM2 in the ipsilateral striatum (E), plasma CXCL1 (F), and plasma IL-6 (G) levels. (H) Similar experiment to , except that mice were sacrificed 180 days after PFF injection and MTK458 dosing (QD, PO) at the indicated concentrations. (I-J) Quantification of (H) is shown. Mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p<0.0001, n.s., not significant.

    Journal: bioRxiv

    Article Title: Pharmacological PINK1 activation ameliorates Pathology in Parkinson’s Disease models

    doi: 10.1101/2023.02.14.528378

    Figure Lengend Snippet: (A) Related to . Immunostaining shows representative images of neurons treated with mPFFs and 2.8 µM MTK458. Primary hippocampal neurons were seeded for immunostaining, treated with mPFFs (0.5 µg/mL) and MTK458 as in (E), and on DIV14 fixed for immunostaining with antibodies against pS129 α-syn, NfH and TUJ1. Scale bar, 50 µm. (B) Mice were challenged with striatal injection of PFFs of PBS. After 2 weeks, ipsilateral or contralateral striatum brain pieces were harvested for sequential extraction with buffers containing increasing amounts of detergent. NP-40 insoluble fractions were analyzed by immunoblot. (C-D) Quantification of (B) is shown. (E-G) Mice were challenged with striatal injection of PBS or PFF, and then dosed with MTK458 for 6 months. Mice were analyzed for TREM2 in the ipsilateral striatum (E), plasma CXCL1 (F), and plasma IL-6 (G) levels. (H) Similar experiment to , except that mice were sacrificed 180 days after PFF injection and MTK458 dosing (QD, PO) at the indicated concentrations. (I-J) Quantification of (H) is shown. Mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p<0.0001, n.s., not significant.

    Article Snippet: Sheep anti-TREM2 polyclonal antibody (R&D systems BAF1729) was coated onto Small Spot Streptavidin 96 well plates (MSD, L45SA-2) as the capture antibody.

    Techniques: Immunostaining, Injection, Extraction, Western Blot, Clinical Proteomics